Abstract: The growing demand for natural, herbal alternatives in oral healthcare has led to the development of an antimicrobial mouthwash using clove (Syzygium aromaticum) and tulsi (Ocimum sanctum) extracts. This study aimed to prepare and evaluate a herbal mouthwash formulated with these extracts for its potential antimicrobial activity. Clove and tulsi are renowned for their bioactive compounds, including eugenol and flavonoids, known to exhibit potent antimicrobial effects. The mouthwash was prepared using standardized extracts of both herbs, ensuring the preservation of their active ingredients. Its antimicrobial efficacy was assessed against common oral pathogen such as E. coli, using well-established microbiological techniques like agar well diffusion. The results indicated significant antimicrobial activity with the herbal mouthwash. Furthermore, the formulation was evaluated for its physical properties, pH, viscosity and stability attributes. This study suggests that the herbal mouthwash containing clove and tulsi extracts can serve as a promising natural alternative for oral hygiene with effective antimicrobial properties, offering an eco-friendly option for consumers seeking alternatives to conventional oral care products.
