Abstract: A natural and efficient substitute for conventional dental care products, herbal toothpaste has both medicinal and aesthetic advantages. This study focuses on creating and testing a polyherbal toothpaste that contains ingredients that are known to have antibacterial and anti-inflammatory qualities, such as ginger, tulsi, clove, acacia gum, peppermint oil, and honey. The toothpaste was prepared using standard procedures and evaluated for physical appearance, pH, spreadability, extrudability, foamability, homogeneity, abrasiveness, moisture content, and antimicrobial activity. The final formulation demonstrated desirable organoleptic and physicochemical characteristics, with a neutral pH (7.5), excellent spreadability (2.9 cm/sec), and no sharp or abrasive particles. Agar well diffusion method confirmed moderate antibacterial activity against Escherichia coli, with a zone of inhibition of 10 mm, outperforming a commercial herbal counterpart. The presence of bioactive phytoconstituents such as eugenol, flavonoids, and menthol contributed to the therapeutic potential of the formulation. These findings support the development of eco-friendly, effective herbal toothpaste for daily oral hygiene.
