Abstract: The interest in researching and developing new antimicrobial agents from different sources to combat microbial resistance has been increasing in recent years. Therefore, greater attention has been paid to screening and testing methods of antimicrobial activity. Several bioassays such as disk-diffusion, well-diffusion and broth or agar dilution are well known and frequently used, but others such as cytofluorometric and bioluminescent methods are not widely used because they require specialized equipment and more reproducibility and standardization evaluation, even though they can provide rapid results of the effects of the antimicrobial agent and better evaluation for reproducibility and standardization. In this review article, an exhaustive list of in vitro-antimicrobial susceptibility testing methods and detailed details on their advantages and limitations are recorded.
